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Transfer
Factor Advanced Formula Russian University Test Results
With two products -
4Life Transfer Factor Classic and
4Life Transfer Factor Plus - that already
set new and higher standards in immune system support - providing boosts
in Natural Killer (NK) cell activity
of 103 and 248 percent - how did 4Life think of trying to improve them?
It started with a dedication to the long-term pursuit of transfer factor
research, which led to the discovery of eggs and another viable source
for transfer factor technology and to provide
the highest quality, most effective products for good health further
drove 4Life to be even better. And finally, it came from the belief that
one of the best ways to improve 4Life Transfer Factor was to broaden the
spectrum of support by combining safe and effective sources of
transfer factors.
4Life researchers and
scientists developed the Transfer Factor
E-XFTM proprietary blend in an effort to further
maximize immune system support using transfer factor molecules.
Transfer factors were taken from both the cow and chicken sources and
combined i hopes of sparking a synergistic-effect which was definitely
acgueved,
4Life Transfer Factor Advanced Formula and
4Life Transfer Factor Plus Advanced Formula
both feature the scientifically proven
Transfer Factor E-XF proprietary blend. Developed exclusively by
4Life using patented and patent-pending technology, the E-XF blend calls
upon the knowledge of two sources, providing an enhanced combined effect
of transfer factors from both cow colostrum and chicken eggs.
Research shows that the
immune building effects of this transfer factor blend are more
effective than that of colostrum or egg sources alone.
In an effort to determine
how powerful the 4Life Transfer Factor
Advanced Formulas are, researchers Calvin McCausland, Ph.D and Emma
Oganova, Ph.D, M.D. designed a study to test
NK cell activity. Dr. Anatoli
Vorobiev at the Russian Academy of Medical Science directed his team in
the independent testing. Using blinded cytotoxicity testing, cancerous
cells were conbined with NK cells from humans and divided into groups of
NK cells activated with transfer factors and
groups of unactivated NK cells.
Samples containing several
different blends of transfer factors sourced from cow colostrum and egg
yolks were incubated with the NK cells
for various time periods, in order to identify the most potent blend
ratio and the optimal activation time of NK cells to provide maximum
health benefits. Comparison of times demonstrated that the activation
time of 48 hours promoted the greatest NK cell response.
Results of this study
conclusively demonstrated the ability of
4Life Transfer Factor Advanced Formula to boost NK cell activity by
283 percent and of 4Life Transfer Factor Plus
Advanced Formula to propel NK cell activity to a remarkable 437
percent above normal immune response (a response established as a
baseline from this study). They also showed that
4Life Transfer Factor Classic increased
NK cell activity by 204 percent when tested at a 48-hour activation
time. Further, results from this scientific experiment showed that
NK cells activated with 4Life
Transfer Factor Plus Advanced Formula killed 97 percent of cancerous
cells, which exceeded the interleukin-2 (IL2) activation kill rate of
88% during the same time period.
The Russian scientists who
conducted this study found the results to be exceptional and requested
further information so their results could be published in professional
journals. One scientist stated: "The
4Life sample [4Life Transfer Factor Plus Advanced Formula] activated NK
cell activity more than the interleukin-2 (IL2) drug used as the
standard. Here, we now refer to your sample as the Golden
Interleukin," said Dr. Kisielevsky, Russian Academy of Medical Sciences.
These outstanding test
results are certain to be reflected in human health through an increase
in immune effectiveness, leading to improved overall health. People
around the world can be confident in the ability of 4Life Transfer
Factor Advanced Formula and 4Life Transfer Factor Plus Advanced Formula
to more effectively modulate immune system function, making it more
response "able" and to provide broad-spectrum support that is unmatched
in any other health supplement today.
Sourced from
http://www.4life.com/TransferFactor/resultsFull.html
Effect of
Transfer Factor Advanced Formulas Containing E-XF Blends on Natural
Killer (NK) Cell Activity
(Russian Cancer Research Centre)
A 4Life Summary of an Independent NK Cell
Study Report by Calvin W. McCausland, Ph.D. and Emma Oganova M.D., Ph.D.
Summary
Objective: To
determine the extent to which Transfer Factor E-XF blends and
4Life
Transfer Factor Plus Advanced Formula increases
Natural Killer (NK) cell
activity above baseline.
Study Design:
The blinded cytotoxicity study was designed by Dr. E. Oganova and Dr. C.
McCausland. The independent testing of the coded samples was done under
the direction of Academician Anatoly Vorobiev, M.D., Russian Academy of
Medical Sciences (RAMS) and the experimental work was conducted by, Dr.
M.V. Kisielevsky, Dr. E.O. Khalturina, at the Russian Cancer Research
Center, RAMS.
Methods: Blood
was obtained from RORC donor station. A standard method of density
gradient was used to isolate mononuclear cells [NK Cells]. The isolated
cells, 60 thousand cells per well, in 100 ml of culture medium, were
introduced into each well of Costar 96 well plates. Then, portions of
coded test samples [Transfer Factor E-XF blends of selected ratios,
4Life Transfer Factor Plus E-XF blend and other samples] were introduced
into the wells at predetermined concentrations (wt./vol.). An
Interleukin-2 (IL-2) standard was used to compare effectiveness. The
preparations were incubated in a CO2-incubator
with a 5% CO2
atmosphere, 100% humidity and 37°C
for 24 or 48 hours. Next 30 thousand K-562 tumor cells (erythroblastic
human leukemia) were introduced
into the the wells. (Thus, the ratio of effector and target cells was
2:1. Each sample was tested in triplicate). The preparations containing
both the effector (NK cells) and target cells (K562 cancer cells) along
with control wells were again incubated for 18-24 hours under the same
incubator conditions. An MTT (dye) solution was used to
spectrophotometricly determine the number of viable cells remaining in
each well. The cytotoxic index (CI) expressed in % is reported for each
sample.
Results: Each of the
samples significantly increased NK cell activity. The Transfer Factor E-XF
Blend (Advanced Formula) resulted in greater NK cell activation (283%)
than the Transfer Factor XF (204%). 4Life Transfer Factor Plus Advanced
Formula increased NK cell activity by 437%. The drug IL-2 increased NK
cell active by 389%. In the
study activation by 4Life Transfer Factor Advanced Formula,
4Life
Transfer Factor Plus Advanced Formula and IL-2 resulted in a kill
rate of K562 cancer cells of 69%, 97% and 88% respectively with the
48 hour incubation period giving the greatest NK cell activation.
Discussion
As previously reported in
earlier publications two 4Life products, 4Life Transfer Factor Classic
and 4Life Transfer Factor Advanced Formula, have been shown to
significantly improve NK cell activation, 103% and 248% respectively.
When the transfer factors from patented egg technology are combined with
bovine transfer factors there is a synergistic activation of NK cell
activity. The improved formulas, 4Life Transfer Factor and 4Life
Transfer Factor Plus Advanced Formulas improve NK cell activation by
283% and 437% respectively.
4Life researchers and
scientists in developing the Advanced Formulas were confident that the
colostrum derived transfer factors when combined with the egg derived
transfer factors (E-XF blends) would enhance the activity and benefit of
the products, but we too were taken by surprise by the magnitude of the
increase. Further research needs to be done to better understand why
this synergism occurs. We are confident these results will translate
into increased benefits to consumers and increased clinical effect in
future studies.
This study was conducted
under the direction of Dr. Anatoli Vorobiev by his colleagues of the
Russian Academy of Medical Science. Using established methods of
cytotoxicity testing, NK cells from humans were combined with cancer
cells and divided into groups of transfer factors activated NK cells and
groups of unactivated NK cells. The objective was to find the bovine and
egg transfer factor blend(s) (E-XF blends) that express the greatest NK
cell activation. The results enable us to identify the most potent
combination that will provide the greatest health benefits.
In the control series of the
experiments for all of the transfer factor samples tested there was no
direct cytotoxic effect on tumor cells at any of the concentrations
tested. When in the absence of the immune NK cells the transfer factors
are combined directly with the cancer cells there is no cytotoxic effect
on the tumor cells.
Results of this study
clearly demonstrated that 4Life Transfer Factor Advanced Formula and
4Life Transfer Factor Plus Advanced Formula boosts NK cell activity. The
independent research scientists who conducted this study found the
results interesting and so exceptional as to request further information
on the identity of 4Life samples so that results could be published in
professional journals. Statements from researchers included the
following: "The 4Life sample activated NK cell activity more than the
Interleukin-2 (IL-2) drug, used as the standard. Here, we now refer to
your sample as the Golden Interleukin," stated Dr. Kisielevsky, Russian
Academy of Medical Sciences.
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