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Background and Rationale
Cancer is the second leading
cause of death in the United States. The rate of nearly every type of
cancer is on the rise. Despite
billions of dollars in research, the morality rate has remained
virtually unchanged since the 1960's using conventional treatments such
as radiation, surgery, and
chemotherapy. Thus, there is considerable interest in using
immune modulation as an adjuvant for
cancer therapy.
Natural killer cells
are particularly important in destroying cancer cells.
Purpose of Study
To determine the in
vitro anti-cancer effects of Transfer FactorTM
and
Transfer Factor PlusTM
using PBMC isolated from human volunteers and assaying the increased
ability of the Natural
Killer cell population to kill K562 erythroleukemic cells.
Methods
1) PBMC killing of K562 (erythroleukemic)
cells
Results
2)
Transfer Factor increased
Natural Killer-cell
killing by 103% and Transfer Factor Plus
by 248%
Conclusions
Transfer Factor
TM
and
Transfer Factor PlusTM
induced immune-based lysis of K562 cells at a level
unprecedented in the director's experience or in the know medical
literature. Since
NK cell function is so crucial in
killing cancer
cells, these products are ideal candidates for cancer adjuvant therapy.
In addition, NK cells form a first line of defense against infections
from viruses and certain other microorganisms. Killing assays against
K562 cells have also been correlated with increased activity against
chronic and acute infections as well.
Darryl See,
MD. & Director, Bioassay Laboratory, Institute of Longevity Medicine (11/5/99)
18800
Delaware Street, Suite 900, Huntington Beach, CA 92648
Dr See
Report with NK information and Cancer Bullets.doc
Transfer Factor Study
N.K. Assay (October 27,
1999)
| Sample Name |
#of Live K562 Cells |
#of Dead K562 Cells |
Mean of Dead cells |
% Increase In Killing |
| Baseline -a |
95.8 |
4.2 |
3.7 |
|
| Baseline -b |
96.9 |
3.1 |
| Positive (PBMC) -a |
95.2 |
4.8 |
5.0 |
|
| Positive (PBMC) -b |
94.8 |
5.2 |
| Negative (Flour) -a |
94.2 |
5.8 |
5.6 |
|
| Negative (Flour) -b |
94.7 |
5.3 |
|
Transfer Factor -a |
87.8 |
12.2 |
11.3 |
103% |
|
Transfer Factor -b |
89.7 |
10.3 |
Transfer Factor Plus Study
N.K. Assay (November 4,
1999)
| Sample Name |
#of Live K562
Cells |
#of Dead K562
Cells |
Mean of Dead
cells |
% Increase In
killing |
| Baseline -a |
99 |
1.3 |
1.6 |
|
| Baseline -b |
98.1 |
1.9 |
| Positive (PBMC)
-a |
95.8 |
42 |
3.6 |
|
| Positive (PBMC)
-b |
97.1 |
2.9 |
| Negative (Flour)
-a |
97.3 |
2.7 |
3.1 |
|
| Negative (Flour)
-b |
96.6 |
3.4 |
|
Transfer Factor
Plus (200 l) -a |
89.7 |
10.3 |
10.6 |
248% |
|
Transfer Factor
Plus (200 l) -b |
69.1 |
10.9 |
|
Transfer Factor
Plus (20 l) -a |
91.8 |
8.2 |
8.0 |
161% |
|
Transfer Factor
Plus (20 l) -b |
92.3 |
7.7 |
Transfer Factor and Transfer
Factor Plus Study
Toxicity Assay
(November 9, 1999)
|
Sample |
Result |
|
Transfer Factor (2 mg) |
No toxicity observed |
| Transfer Factor (200 g) |
No toxicity observed |
| Transfer Factor Plus (2 mg) |
No toxicity observed |
| Transfer Factor Plus (200 g) |
No toxicity observed |
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Dr See Report with NK
information and Cancer Bullets.doc
Nutraceutical Induced
Increase in Natural Killer Cell (NK) Activity
Purpose of Study
To determine the in vitro
Natural Killer Cell
(NK) activation effects of 4Life Transfer
Factor and Transfer Factor Plus using
peripheral blood mononuclear cells (PBMC) isolated from human volunteers
and assaying the increased ability of the NK cell population to kill
K562 erthroleukemic cells.
Conclusion
4Life Transfer Factor and Transfer Factor Plus induced immune-based
lysis (breakdown) of K562 cells at a level unprecedented in the
director's experience or in any known medical literature. Since
Natural Killer cell
function is so crucial in killing dangerous Cells, these products
are ideal candidates for human and animal immune support.
|
Nutraceutical
Tested |
Percent (%) Rise in
NK Cell Activity |
|
4Life Transfer Factor Plus |
248 % |
|
4Life Transfer Factor |
103 % |
| IP6 (highest of group
Previously tested) |
49 % |
| Plant Polysaccharide
Formula |
48 % |
| Echinacea |
47 % |
| Shitake Mushroom |
42 % |
| Cordecep Formula |
28 % |
| Bovine Colostrum |
23 % |
| Phytonutrient Formula
with Garlic |
21 % |
| Endocrine System Formula |
16 % |
| Concentrate (acemannan) |
15 % |
| Noni (Morinda citrafolia) |
15 % |
Some of these products reflect an average
of different brands.
Baseline activity is the measure of the
unassisted ability of white blood cells to kill K562 leukemia (cancer)
cells. Natural Killer
Cells are the body's first line of defense against cancers and
viruses. TheTo increase in the
ability of white blood cells to kill K562 cancer cells is directly
related to the increase in Natural Killer Cell activity.
This study does not claim nor should it
be interpreted to cure, prevent or mitigate any serious disease. The
results have been reported as they were received. If you have a serious
illness, see your Health Professional.
Understanding the Immune System
Natural
Killer Cells
Natural Killer (NK) Cells
are yet another type of lethal lymphocyte. Like cytotoxic T cells, they
contain granules filled with potent chemicals. They are called "natural"
killers because they, unlike cytotoxic T cells, do not need to recognize
a specific antigen before swinging into action. They target tumor cells
and protect against a wide variety of infectious microbes. In several
immunodeficiency diseases, including
AIDS,
natural killer cell function
is abnormal. Natural killer cells may also contribute to immunoregulation by secreting high levels of influential lymphokines.
Both cytotoxic T cells and
natural killer cells kill on contact. The killer binds to its target,
aims its weapons, and then delivers a lethal burst of chemicals that
produces holes in the target cell's membrane. Fluids seep in and leak
out, and the cell bursts.
Natural Killer Cells
The back-bone of the
human immune system.
The following links provide
information on research related to natural killer cells - one of the
main types of white blood cells which work as part of the human
immune
system. Natural killer cells are being found to play a primary role
in preventing and removing cancer cells in the body, removing many types
of viruses (including herpes and measles) and have been found low in
women with endometriosis.
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